A National Cancer Institute-designated Comprehensive Cancer Center

Make an appointment: 800-826-HOPE
Pathology Bookmark and Share

Pathology

Led by pathologists renowned for diagnostic excellence, the Department of Pathology at City of Hope combines state-of-the-art laboratories equipped with the latest diagnostic techniques and advanced instrumentation with superior investigative skills to accurately and rapidly identify even the rarest and most complex diseases. Our experts provide comprehensive services, from initial diagnosis to monitoring of prognostic indicators throughout the treatment process. Our team also maintains a blood bank and offers a full complement of services relating to blood and bone marrow donors.
 
Pathology Consultation Services
Our Pathology Consultation Services offers a comprehensive program to fulfill all pathology needs. Physicians at City of Hope and from around the world choose to submit their biopsy samples here because our laboratories are of the highest caliber and our team of pathologists are recognized experts in diagnosing and monitoring disease.
 
Our team takes a coordinated and cooperative approach, and final interpretations are only made at a daily consensus conference of senior staff, reinforcing the validity of the initial pathologist’s opinion and minimizing any possibility of error.
 
Rapid, Integrated Results at Competitive Prices
Our highly efficient support staff – from receptionists to transcriptionists to managers – helps deliver complete, accurate reports quickly to the requesting physician. The results of multiple tests are combined into one integrated report, for quick, clear access to priority tests. In most cases, results are obtained within 24 to 48 hours, with automatic faxing capabilities once the reports are signed out. As a nonprofit institution, we are able to offer highly competitive prices for our services. Our regular rates are low, and contracts can be negotiated for prospective high-volume clients. Professional revenues generated are used to support the department’s research activities.

 

 

Pathology Case Submission

Cases submitted to a specific pathologist will be read by the designated party. Otherwise we will assign the case to an appropriate investigator.

Always include the patient's clinical history along with referring physician's name, phone and fax numbers on the requisition form.

Send cases to:

City of Hope
Attn: Anatomic Pathology
1500 E. Duarte Road
Duarte, CA 91010

 

 

Pathology Customer Service

Customer and Financial Services
We are committed to providing superior pathology consultation services and supporting your needs in an increasingly competitive and cost-conscious medical marketplace.
 
To that end, we endeavor to complete your case as quickly as technically possible while maintaining our high quality standards.
 
For your convenience, we will bill third party payers. Simply complete the insurance information on the requisition form. In the absence of complete third party information, the referring pathologist or facility will be billed.
 
Unfortunately, due to Medicare regulations, we are not able to bill a third party payer if the patient is an inpatient at the referring facility. In this case, the referring institution will be billed. However, we do offer contract prices for these cases with highly competitive rates.
 
Specimen Collection
We offer free courier services for facilities located in the Southern California area. For facilities outside of Southern California, please call 626-256-HOPE (4673), ext. 63875, to coordinate pick up.

 

 

Pathology Laboratories

Immunochistochemistry
Our immunohistochemistry laboratory includes three high-volume automated immunostainers using highly advanced epitope retrieval techniques.  We offer complete immunohistochemistry characterization of surgical and cytologic specimens, including estrogen and progesterone receptors and over 100 other monoclonal and polyclonal antibodies. Each year, our hematopathology laboratory performs cytochemistry on more than 1,000 cases of acute leukemia and processes thousands of bone marrow specimens annually.
 
Flow Cytometry
Our flow cytometry laboratory uses high-capacity instruments to analyze thousands of samples annually to measure a variety of parameters, including cell surface antigens. The presence or absence of certain cell surface antigens has shown great prognostic value in various neoplasia, where treatment protocols can be optimized by customizing the type of treatment based on the types of cell surface antigens present. We provide comprehensive cytochemical and immunophenotypic characterization of leukemias and lymphomas; B and T cell subset analysis for immunodeficiencies; stem cell counts; solid tumor DNA content and cell cycle analysis.
 
Cytogenetics
Every year, the cytogenetics laboratory processes a heavy volume of neoplastic specimens, utilizing both conventional cytogenetics as well as fluorescence in situ hybridization (FISH). FISH has become the recognized gold standard in determining the likely course of disease and appropriate treatment in leukemias such as chronic lymphocytic.
 
Molecular Biology
Our molecular biology laboratory performs Southern blot hybridization, polymerase chain reaction, and in situ hybridization studies for B and T cell gene rearrangements, many different chromosomal translocations for lymphoma and leukemia, including t(14;18), t(9;22), t(15;17), and Epstein-Barr virus. Many of the molecular tests can be performed on paraffin-embedded tissue.
 
Laboratory accreditation, certification and licensing
The Consultation Services Laboratories at City of Hope are fully accredited by the College of American Pathologists (CAP #22900-01), certified under the Clinical Laboratory Improvements Amendments of 1988 (CLIA '88, ID No. 05D0665695) and licensed by the state of California (CLF 268).

The laboratories participate in the CAP's external proficiency survey program for flow cytometry, immunophenotyping, immunohistochemistry, cytogenetics and molecular oncology. Specific details on our survey performance are available upon request.
 

Antibody Library

Actin
AFP (alpha-fetprotein)
ALK protein
bcl-1
bcl-2
bcl-6
BerEp4
BG8
Calcitonin
Calretinin
CD1
CD2
CD3
CD4
CD5
CD7
CD8
CD10
CD15
CD16
CD20
CD21
CD23
CD30
CD31
CD34
CD35
CD343
CD45RA
CD45/45RB
CD45RO
CD56
CD57
CD68
CD79A
CD99
CD117 (c-kit)
CD138
CEA-Gold1
CEA-Gold5
c-erb-2
Chromogranin
Collagen-IV
DBA.44
Desmin
EBV-LMP
EMA (epithelial membrane  antigen)
ER (estrogen receptor)
Factor XIII (vWF)
FASCIN
GCDFP-15
GFAP
Granzyme B
HBME-1
HCG
Helico
Hemoglobin A
HLA-DR
HMB45
Inhibin
Kappa
Keratin 5/6
Keratin 8/18
Keratin 7
Keratin 8
Keratin 14
Keratin 19 (AE-1)
Keratin 20
Keratin cocktail (pan keratin)
34 βE 12 (HMW Keratin)
Ki-67
Lambda
Lysozyme
Melan-A
mes-13
MOC
MPX (myeloperoxidase)
NF (neurofilament)
p53
PacP (prostate acid phosphatase)
Perforin
PLAP (placential alkaline phosphatase)
PR (progesterone receptor)
Prolactin
PSA (prostate specifice antigen)
S100
SMA (smooth muscle actin)
Surfactant
Synaptophysin
Tdt
TGB (thyroglobin)
Thrombomodulin
TIA-1
TRAP
Tryptase
TTF-1
Villin
Vimentin
vWF (factor VIII)

Specimen Draw and Shipping Requirements

Cytogenetics
Results for STATs, pre-BMT's and newly diagnosed leukemias are available within seven days. All others are available in seven to 10 days but our goal is to report results as soon as they are available. Complex studies may require additional days. Specimens are accepted Monday through Friday, 8 a.m. to 5:30 p.m.
 
Drawing specifications
  • Leukemia and myelodysplastic syndromes: A bone marrow aspirate is the specimen of choice in most cases of leukemia, myelodysplastic and myeloproliferative disorders.
  • Leukemia cytogenetics: Obtain 1 to 2 ml of bone marrow (minimum 0.5 ml) in a heparinized syringe (approximately 500 USP sodium heparin per ml of specimen) and transfer the specimen into a green top sodium heparin tube. Keep at room temperature and ship as soon as possible.
  • Leukemic peripheral blood: Draw 5 to 20 ml of peripheral blood into a green top sodium heparin tube. Mix well by inverting. Keep at room temperature and ship as soon as possible.
  • Lymphoma and solid tumor cytogenetics: Bone marrow core biopsies should be placed in HBSS or RPMI medium.
 
Flow cytometry / molecular diagnostics
The laboratory is open Monday through Friday, 8 a.m. to 5 p.m.
 
Drawing specifications
  • Peripheral blood: For leukemia and lymphoma panels, draw six green top tubes (sodium herapin) and one purple top tube. Maintain at room temperature and ship as soon as possible. ACD tubes are acceptable. Sodium lithium should not be used.
  • Bone marrow: Draw at least one purple top tube, maintain at room temperature and ship as soon as possible. ACD and sodium heparin tubes are acceptable.
 
 

Pathology Team

Support this program

It takes the help of a lot of caring people to make hope a reality for our patients.  City of Hope was founded by individuals’ philanthropic efforts 100 years ago. Their efforts — and those of our supporters today — have built the foundation for the care we provide and the research we conduct. It enables City of Hope to strive for new breakthroughs and better therapies, ultimately helping more people enjoy longer, better lives.

For more information on supporting this specific program, please contact our Donor Relations Department at 800-667-5310 or developmentrelations@coh.org. Or, to make a gift that supports all the research at City of Hope, donate online now.
 
We thank you for your support.
 
 
 

Pathology

Pathology

Led by pathologists renowned for diagnostic excellence, the Department of Pathology at City of Hope combines state-of-the-art laboratories equipped with the latest diagnostic techniques and advanced instrumentation with superior investigative skills to accurately and rapidly identify even the rarest and most complex diseases. Our experts provide comprehensive services, from initial diagnosis to monitoring of prognostic indicators throughout the treatment process. Our team also maintains a blood bank and offers a full complement of services relating to blood and bone marrow donors.
 
Pathology Consultation Services
Our Pathology Consultation Services offers a comprehensive program to fulfill all pathology needs. Physicians at City of Hope and from around the world choose to submit their biopsy samples here because our laboratories are of the highest caliber and our team of pathologists are recognized experts in diagnosing and monitoring disease.
 
Our team takes a coordinated and cooperative approach, and final interpretations are only made at a daily consensus conference of senior staff, reinforcing the validity of the initial pathologist’s opinion and minimizing any possibility of error.
 
Rapid, Integrated Results at Competitive Prices
Our highly efficient support staff – from receptionists to transcriptionists to managers – helps deliver complete, accurate reports quickly to the requesting physician. The results of multiple tests are combined into one integrated report, for quick, clear access to priority tests. In most cases, results are obtained within 24 to 48 hours, with automatic faxing capabilities once the reports are signed out. As a nonprofit institution, we are able to offer highly competitive prices for our services. Our regular rates are low, and contracts can be negotiated for prospective high-volume clients. Professional revenues generated are used to support the department’s research activities.

 

 

Pathology Case Submission

Pathology Case Submission

Cases submitted to a specific pathologist will be read by the designated party. Otherwise we will assign the case to an appropriate investigator.

Always include the patient's clinical history along with referring physician's name, phone and fax numbers on the requisition form.

Send cases to:

City of Hope
Attn: Anatomic Pathology
1500 E. Duarte Road
Duarte, CA 91010

 

 

Pathology Customer Service

Pathology Customer Service

Customer and Financial Services
We are committed to providing superior pathology consultation services and supporting your needs in an increasingly competitive and cost-conscious medical marketplace.
 
To that end, we endeavor to complete your case as quickly as technically possible while maintaining our high quality standards.
 
For your convenience, we will bill third party payers. Simply complete the insurance information on the requisition form. In the absence of complete third party information, the referring pathologist or facility will be billed.
 
Unfortunately, due to Medicare regulations, we are not able to bill a third party payer if the patient is an inpatient at the referring facility. In this case, the referring institution will be billed. However, we do offer contract prices for these cases with highly competitive rates.
 
Specimen Collection
We offer free courier services for facilities located in the Southern California area. For facilities outside of Southern California, please call 626-256-HOPE (4673), ext. 63875, to coordinate pick up.

 

 

Pathology Laboratories

Pathology Laboratories

Immunochistochemistry
Our immunohistochemistry laboratory includes three high-volume automated immunostainers using highly advanced epitope retrieval techniques.  We offer complete immunohistochemistry characterization of surgical and cytologic specimens, including estrogen and progesterone receptors and over 100 other monoclonal and polyclonal antibodies. Each year, our hematopathology laboratory performs cytochemistry on more than 1,000 cases of acute leukemia and processes thousands of bone marrow specimens annually.
 
Flow Cytometry
Our flow cytometry laboratory uses high-capacity instruments to analyze thousands of samples annually to measure a variety of parameters, including cell surface antigens. The presence or absence of certain cell surface antigens has shown great prognostic value in various neoplasia, where treatment protocols can be optimized by customizing the type of treatment based on the types of cell surface antigens present. We provide comprehensive cytochemical and immunophenotypic characterization of leukemias and lymphomas; B and T cell subset analysis for immunodeficiencies; stem cell counts; solid tumor DNA content and cell cycle analysis.
 
Cytogenetics
Every year, the cytogenetics laboratory processes a heavy volume of neoplastic specimens, utilizing both conventional cytogenetics as well as fluorescence in situ hybridization (FISH). FISH has become the recognized gold standard in determining the likely course of disease and appropriate treatment in leukemias such as chronic lymphocytic.
 
Molecular Biology
Our molecular biology laboratory performs Southern blot hybridization, polymerase chain reaction, and in situ hybridization studies for B and T cell gene rearrangements, many different chromosomal translocations for lymphoma and leukemia, including t(14;18), t(9;22), t(15;17), and Epstein-Barr virus. Many of the molecular tests can be performed on paraffin-embedded tissue.
 
Laboratory accreditation, certification and licensing
The Consultation Services Laboratories at City of Hope are fully accredited by the College of American Pathologists (CAP #22900-01), certified under the Clinical Laboratory Improvements Amendments of 1988 (CLIA '88, ID No. 05D0665695) and licensed by the state of California (CLF 268).

The laboratories participate in the CAP's external proficiency survey program for flow cytometry, immunophenotyping, immunohistochemistry, cytogenetics and molecular oncology. Specific details on our survey performance are available upon request.
 

Antibody Library

Antibody Library

Actin
AFP (alpha-fetprotein)
ALK protein
bcl-1
bcl-2
bcl-6
BerEp4
BG8
Calcitonin
Calretinin
CD1
CD2
CD3
CD4
CD5
CD7
CD8
CD10
CD15
CD16
CD20
CD21
CD23
CD30
CD31
CD34
CD35
CD343
CD45RA
CD45/45RB
CD45RO
CD56
CD57
CD68
CD79A
CD99
CD117 (c-kit)
CD138
CEA-Gold1
CEA-Gold5
c-erb-2
Chromogranin
Collagen-IV
DBA.44
Desmin
EBV-LMP
EMA (epithelial membrane  antigen)
ER (estrogen receptor)
Factor XIII (vWF)
FASCIN
GCDFP-15
GFAP
Granzyme B
HBME-1
HCG
Helico
Hemoglobin A
HLA-DR
HMB45
Inhibin
Kappa
Keratin 5/6
Keratin 8/18
Keratin 7
Keratin 8
Keratin 14
Keratin 19 (AE-1)
Keratin 20
Keratin cocktail (pan keratin)
34 βE 12 (HMW Keratin)
Ki-67
Lambda
Lysozyme
Melan-A
mes-13
MOC
MPX (myeloperoxidase)
NF (neurofilament)
p53
PacP (prostate acid phosphatase)
Perforin
PLAP (placential alkaline phosphatase)
PR (progesterone receptor)
Prolactin
PSA (prostate specifice antigen)
S100
SMA (smooth muscle actin)
Surfactant
Synaptophysin
Tdt
TGB (thyroglobin)
Thrombomodulin
TIA-1
TRAP
Tryptase
TTF-1
Villin
Vimentin
vWF (factor VIII)

Specimen Draw and Shipping Requirements

Specimen Draw and Shipping Requirements

Cytogenetics
Results for STATs, pre-BMT's and newly diagnosed leukemias are available within seven days. All others are available in seven to 10 days but our goal is to report results as soon as they are available. Complex studies may require additional days. Specimens are accepted Monday through Friday, 8 a.m. to 5:30 p.m.
 
Drawing specifications
  • Leukemia and myelodysplastic syndromes: A bone marrow aspirate is the specimen of choice in most cases of leukemia, myelodysplastic and myeloproliferative disorders.
  • Leukemia cytogenetics: Obtain 1 to 2 ml of bone marrow (minimum 0.5 ml) in a heparinized syringe (approximately 500 USP sodium heparin per ml of specimen) and transfer the specimen into a green top sodium heparin tube. Keep at room temperature and ship as soon as possible.
  • Leukemic peripheral blood: Draw 5 to 20 ml of peripheral blood into a green top sodium heparin tube. Mix well by inverting. Keep at room temperature and ship as soon as possible.
  • Lymphoma and solid tumor cytogenetics: Bone marrow core biopsies should be placed in HBSS or RPMI medium.
 
Flow cytometry / molecular diagnostics
The laboratory is open Monday through Friday, 8 a.m. to 5 p.m.
 
Drawing specifications
  • Peripheral blood: For leukemia and lymphoma panels, draw six green top tubes (sodium herapin) and one purple top tube. Maintain at room temperature and ship as soon as possible. ACD tubes are acceptable. Sodium lithium should not be used.
  • Bone marrow: Draw at least one purple top tube, maintain at room temperature and ship as soon as possible. ACD and sodium heparin tubes are acceptable.
 
 

Pathology Team

Pathology Team

Support This Program

Support this program

It takes the help of a lot of caring people to make hope a reality for our patients.  City of Hope was founded by individuals’ philanthropic efforts 100 years ago. Their efforts — and those of our supporters today — have built the foundation for the care we provide and the research we conduct. It enables City of Hope to strive for new breakthroughs and better therapies, ultimately helping more people enjoy longer, better lives.

For more information on supporting this specific program, please contact our Donor Relations Department at 800-667-5310 or developmentrelations@coh.org. Or, to make a gift that supports all the research at City of Hope, donate online now.
 
We thank you for your support.
 
 
 
Quick Links
With Cancer, Expertise Matters

 
Cancer patients need to have confidence in their treatment plans by exploring all possible options. Often that means they should get a second opinion.  For these four patients, getting a second opinion from experts at City of Hope was life-saving.
Supportive Care Calendar
 
With MyCityofHope your health information is right at your fingertips, anywhere, any time.


NEWS & UPDATES
  • The body’s immune system is usually adept at attacking outside invaders such as bacteria and viruses. But because cancer originates from the body’s own cells, the immune system can fail to see it as foreign. As a result, the body’s most powerful ally can remain largely idle against cancer as the disease progres...
  • On Jan. 1, 2015, five City of Hope patients who have journeyed through cancer will welcome the new year with their loved ones atop City of Hope’s Tournament of Roses Parade float. The theme of the float is “Made Possible by HOPE.” The theme of the parade is “Inspiring Stories.” Her...
  • Are you thinking about switching from traditional cigarettes to e-cigarettes for the Great American Smokeout? Are you thinking that might be a better option than the traditional quit-smoking route? Think again. For lung expert Brian Tiep, M.D., the dislike and distrust he feels for e-cigs comes down to this: Th...
  • Hematologist Robert Chen, M.D., is boosting scientific discovery at City of Hope and, by extension, across the nation. Just ask the National Cancer Institute. The institution recently awarded Chen the much-sought-after Clinical Investigator Team Leadership Award for boosting scientific discovery at City of Hope...
  • Great strides have been made in treating cancer – including lung cancer – but by the time people show symptoms of the disease, the cancer has usually advanced. That’s because, at early stages, lung cancer has no symptoms. Only recently has lung cancer screening become an option. (Read more about the risks...
  • Identifying cures for currently incurable diseases and providing patients with safe, fast and potentially lifesaving treatments is the focus of City of Hope’s new Alpha Clinic for Cell Therapy and Innovation (ACT-I). The clinic is funded by an $8 million, five-year grant from the California Institute for Regene...
  • Cancer is a couple’s disease. It affects not just the person diagnosed, but his or her partner as well. It also affects the ability of both people to communicate effectively. The Couples Coping with Cancer Together program at City of Hope teaches couples how to communicate and solve problems as a unit. He...
  • Chemotherapy drugs work by either killing cancer cells or by stopping them from multiplying, that is, dividing. Some of the more powerful drugs used to treat cancer do their job by interfering with the cancer cells’ DNA and RNA growth, preventing them from copying themselves and dividing. Such drugs, however, l...
  • During October, everything seems to turn pink – clothing, the NFL logo, tape dispensers, boxing gloves, blenders, soup cans, you name it – in order to raise awareness for what many believe is the most dangerous cancer that affects women: breast cancer. But, in addition to thinking pink, women should...
  • In February 2003, when she was only 16 months old, Maya Gallardo was diagnosed with acute myelogenous leukemia (AML) and, to make matters much worse, pneumonia. The pneumonia complicated what was already destined to be grueling treatment regimen. To assess the extent of her illness, Maya had to endure a spinal ...
  • Former smokers age 55 to 74 who rely on Medicare for health care services have just received a long-hoped-for announcement. Under a proposed decision from the Centers for Medicare and Medicaid Services, they’ll now have access to lung cancer screening with a low-dose CT scan. The proposed decision, announ...
  • City of Hope has a longstanding commitment to combating diabetes, a leading national and global health threat. Already, it’s scored some successes, from research that led to the development of synthetic human insulin – still used by millions of patients – to potentially lifesaving islet cell transplants. Diabet...
  • Dee Hunt never smoked. Neither did her five sisters and brothers. They didn’t have exposure to radon or asbestos, either. That didn’t prevent every one of them from being diagnosed with lung cancer. Their parents were smokers, but they’d all left home more than 30 years before any of them were diagn...
  • They may not talk about it, but women with cancers in the pelvic region, such as cervical cancer, bladder cancer and uterine cancer, often have problems controlling their urine, bowel or flatus. Although they may feel isolated, they’re far from alone. Many other women have such problems, too. In fact, nea...
  • Cancer that spreads to the liver poses a significant threat to patients, and a great challenge to surgeons. The organ’s anatomical complexity and its maze of blood vessels make removal of tumors difficult, even for specialized liver cancer surgeons. Following chemotherapy, the livers of cancer patients are not ...