A National Cancer Institute-designated Comprehensive Cancer Center

Make an appointment: 800-826-HOPE
Pathology Bookmark and Share

Pathology
Led by pathologists renowned for diagnostic excellence, the Department of Pathology at City of Hope combines state-of-the-art laboratories equipped with the latest diagnostic techniques and advanced instrumentation with superior investigative skills to accurately and rapidly identify even the rarest and most complex diseases. Our experts provide comprehensive services, from initial diagnosis to monitoring of prognostic indicators throughout the treatment process. Our team also maintains a blood bank and offers a full complement of services relating to blood and bone marrow donors.
 
Pathology Consultation Services
Our Pathology Consultation Services offers a comprehensive program to fulfill all pathology needs. Physicians at City of Hope and from around the world choose to submit their biopsy samples here because our laboratories are of the highest caliber and our team of pathologists are recognized experts in diagnosing and monitoring disease.
 
Our team takes a coordinated and cooperative approach, and final interpretations are only made at a daily consensus conference of senior staff, reinforcing the validity of the initial pathologist’s opinion and minimizing any possibility of error.
 
Rapid, Integrated Results at Competitive Prices
Our highly efficient support staff – from receptionists to transcriptionists to managers – helps deliver complete, accurate reports quickly to the requesting physician. The results of multiple tests are combined into one integrated report, for quick, clear access to priority tests. In most cases, results are obtained within 24 to 48 hours, with automatic faxing capabilities once the reports are signed out. As a nonprofit institution, we are able to offer highly competitive prices for our services. Our regular rates are low, and contracts can be negotiated for prospective high-volume clients. Professional revenues generated are used to support the department’s research activities.

 

 
Cases submitted to a specific pathologist will be read by the designated party. Otherwise we will assign the case to an appropriate investigator.

Always include the patient's clinical history along with referring physician's name, phone and fax numbers on the requisition form.

Send cases to:

City of Hope
Attn: Anatomic Pathology
1500 E. Duarte Road
Duarte, CA 91010

 

 
Customer and Financial Services
We are committed to providing superior pathology consultation services and supporting your needs in an increasingly competitive and cost-conscious medical marketplace.
 
To that end, we endeavor to complete your case as quickly as technically possible while maintaining our high quality standards.
 
For your convenience, we will bill third party payers. Simply complete the insurance information on the requisition form. In the absence of complete third party information, the referring pathologist or facility will be billed.
 
Unfortunately, due to Medicare regulations, we are not able to bill a third party payer if the patient is an inpatient at the referring facility. In this case, the referring institution will be billed. However, we do offer contract prices for these cases with highly competitive rates.
 
Specimen Collection
We offer free courier services for facilities located in the Southern California area. For facilities outside of Southern California, please call 626-256-HOPE (4673), ext. 63875, to coordinate pick up.

 

 
Immunochistochemistry
Our immunohistochemistry laboratory includes three high-volume automated immunostainers using highly advanced epitope retrieval techniques.  We offer complete immunohistochemistry characterization of surgical and cytologic specimens, including estrogen and progesterone receptors and over 100 other monoclonal and polyclonal antibodies. Each year, our hematopathology laboratory performs cytochemistry on more than 1,000 cases of acute leukemia and processes thousands of bone marrow specimens annually.
 
Flow Cytometry
Our flow cytometry laboratory uses high-capacity instruments to analyze thousands of samples annually to measure a variety of parameters, including cell surface antigens. The presence or absence of certain cell surface antigens has shown great prognostic value in various neoplasia, where treatment protocols can be optimized by customizing the type of treatment based on the types of cell surface antigens present. We provide comprehensive cytochemical and immunophenotypic characterization of leukemias and lymphomas; B and T cell subset analysis for immunodeficiencies; stem cell counts; solid tumor DNA content and cell cycle analysis.
 
Cytogenetics
Every year, the cytogenetics laboratory processes a heavy volume of neoplastic specimens, utilizing both conventional cytogenetics as well as fluorescence in situ hybridization (FISH). FISH has become the recognized gold standard in determining the likely course of disease and appropriate treatment in leukemias such as chronic lymphocytic.
 
Molecular Biology
Our molecular biology laboratory performs Southern blot hybridization, polymerase chain reaction, and in situ hybridization studies for B and T cell gene rearrangements, many different chromosomal translocations for lymphoma and leukemia, including t(14;18), t(9;22), t(15;17), and Epstein-Barr virus. Many of the molecular tests can be performed on paraffin-embedded tissue.
 
Laboratory accreditation, certification and licensing
The Consultation Services Laboratories at City of Hope are fully accredited by the College of American Pathologists (CAP #22900-01), certified under the Clinical Laboratory Improvements Amendments of 1988 (CLIA '88, ID No. 05D0665695) and licensed by the state of California (CLF 268).

The laboratories participate in the CAP's external proficiency survey program for flow cytometry, immunophenotyping, immunohistochemistry, cytogenetics and molecular oncology. Specific details on our survey performance are available upon request.
 
Actin
AFP (alpha-fetprotein)
ALK protein
bcl-1
bcl-2
bcl-6
BerEp4
BG8
Calcitonin
Calretinin
CD1
CD2
CD3
CD4
CD5
CD7
CD8
CD10
CD15
CD16
CD20
CD21
CD23
CD30
CD31
CD34
CD35
CD343
CD45RA
CD45/45RB
CD45RO
CD56
CD57
CD68
CD79A
CD99
CD117 (c-kit)
CD138
CEA-Gold1
CEA-Gold5
c-erb-2
Chromogranin
Collagen-IV
DBA.44
Desmin
EBV-LMP
EMA (epithelial membrane  antigen)
ER (estrogen receptor)
Factor XIII (vWF)
FASCIN
GCDFP-15
GFAP
Granzyme B
HBME-1
HCG
Helico
Hemoglobin A
HLA-DR
HMB45
Inhibin
Kappa
Keratin 5/6
Keratin 8/18
Keratin 7
Keratin 8
Keratin 14
Keratin 19 (AE-1)
Keratin 20
Keratin cocktail (pan keratin)
34 βE 12 (HMW Keratin)
Ki-67
Lambda
Lysozyme
Melan-A
mes-13
MOC
MPX (myeloperoxidase)
NF (neurofilament)
p53
PacP (prostate acid phosphatase)
Perforin
PLAP (placential alkaline phosphatase)
PR (progesterone receptor)
Prolactin
PSA (prostate specifice antigen)
S100
SMA (smooth muscle actin)
Surfactant
Synaptophysin
Tdt
TGB (thyroglobin)
Thrombomodulin
TIA-1
TRAP
Tryptase
TTF-1
Villin
Vimentin
vWF (factor VIII)
Cytogenetics
Results for STATs, pre-BMT's and newly diagnosed leukemias are available within seven days. All others are available in seven to 10 days but our goal is to report results as soon as they are available. Complex studies may require additional days. Specimens are accepted Monday through Friday, 8 a.m. to 5:30 p.m.
 
Drawing specifications
  • Leukemia and myelodysplastic syndromes: A bone marrow aspirate is the specimen of choice in most cases of leukemia, myelodysplastic and myeloproliferative disorders.
  • Leukemia cytogenetics: Obtain 1 to 2 ml of bone marrow (minimum 0.5 ml) in a heparinized syringe (approximately 500 USP sodium heparin per ml of specimen) and transfer the specimen into a green top sodium heparin tube. Keep at room temperature and ship as soon as possible.
  • Leukemic peripheral blood: Draw 5 to 20 ml of peripheral blood into a green top sodium heparin tube. Mix well by inverting. Keep at room temperature and ship as soon as possible.
  • Lymphoma and solid tumor cytogenetics: Bone marrow core biopsies should be placed in HBSS or RPMI medium.
 
Flow cytometry / molecular diagnostics
The laboratory is open Monday through Friday, 8 a.m. to 5 p.m.
 
Drawing specifications
  • Peripheral blood: For leukemia and lymphoma panels, draw six green top tubes (sodium herapin) and one purple top tube. Maintain at room temperature and ship as soon as possible. ACD tubes are acceptable. Sodium lithium should not be used.
  • Bone marrow: Draw at least one purple top tube, maintain at room temperature and ship as soon as possible. ACD and sodium heparin tubes are acceptable.
 
 

Pathology Team

It takes the help of a lot of caring people to make hope a reality for our patients.  City of Hope was founded by individuals’ philanthropic efforts 100 years ago. Their efforts — and those of our supporters today — have built the foundation for the care we provide and the research we conduct. It enables City of Hope to strive for new breakthroughs and better therapies, ultimately helping more people enjoy longer, better lives.

For more information on supporting this specific program, please contact our Donor Relations Department at 800-667-5310 or developmentrelations@coh.org. Or, to make a gift that supports all the research at City of Hope, donate online now.
 
We thank you for your support.
 
 
 

Pathology

Pathology
Led by pathologists renowned for diagnostic excellence, the Department of Pathology at City of Hope combines state-of-the-art laboratories equipped with the latest diagnostic techniques and advanced instrumentation with superior investigative skills to accurately and rapidly identify even the rarest and most complex diseases. Our experts provide comprehensive services, from initial diagnosis to monitoring of prognostic indicators throughout the treatment process. Our team also maintains a blood bank and offers a full complement of services relating to blood and bone marrow donors.
 
Pathology Consultation Services
Our Pathology Consultation Services offers a comprehensive program to fulfill all pathology needs. Physicians at City of Hope and from around the world choose to submit their biopsy samples here because our laboratories are of the highest caliber and our team of pathologists are recognized experts in diagnosing and monitoring disease.
 
Our team takes a coordinated and cooperative approach, and final interpretations are only made at a daily consensus conference of senior staff, reinforcing the validity of the initial pathologist’s opinion and minimizing any possibility of error.
 
Rapid, Integrated Results at Competitive Prices
Our highly efficient support staff – from receptionists to transcriptionists to managers – helps deliver complete, accurate reports quickly to the requesting physician. The results of multiple tests are combined into one integrated report, for quick, clear access to priority tests. In most cases, results are obtained within 24 to 48 hours, with automatic faxing capabilities once the reports are signed out. As a nonprofit institution, we are able to offer highly competitive prices for our services. Our regular rates are low, and contracts can be negotiated for prospective high-volume clients. Professional revenues generated are used to support the department’s research activities.

 

 

Pathology Case Submission

Cases submitted to a specific pathologist will be read by the designated party. Otherwise we will assign the case to an appropriate investigator.

Always include the patient's clinical history along with referring physician's name, phone and fax numbers on the requisition form.

Send cases to:

City of Hope
Attn: Anatomic Pathology
1500 E. Duarte Road
Duarte, CA 91010

 

 

Pathology Customer Service

Customer and Financial Services
We are committed to providing superior pathology consultation services and supporting your needs in an increasingly competitive and cost-conscious medical marketplace.
 
To that end, we endeavor to complete your case as quickly as technically possible while maintaining our high quality standards.
 
For your convenience, we will bill third party payers. Simply complete the insurance information on the requisition form. In the absence of complete third party information, the referring pathologist or facility will be billed.
 
Unfortunately, due to Medicare regulations, we are not able to bill a third party payer if the patient is an inpatient at the referring facility. In this case, the referring institution will be billed. However, we do offer contract prices for these cases with highly competitive rates.
 
Specimen Collection
We offer free courier services for facilities located in the Southern California area. For facilities outside of Southern California, please call 626-256-HOPE (4673), ext. 63875, to coordinate pick up.

 

 

Pathology Laboratories

Immunochistochemistry
Our immunohistochemistry laboratory includes three high-volume automated immunostainers using highly advanced epitope retrieval techniques.  We offer complete immunohistochemistry characterization of surgical and cytologic specimens, including estrogen and progesterone receptors and over 100 other monoclonal and polyclonal antibodies. Each year, our hematopathology laboratory performs cytochemistry on more than 1,000 cases of acute leukemia and processes thousands of bone marrow specimens annually.
 
Flow Cytometry
Our flow cytometry laboratory uses high-capacity instruments to analyze thousands of samples annually to measure a variety of parameters, including cell surface antigens. The presence or absence of certain cell surface antigens has shown great prognostic value in various neoplasia, where treatment protocols can be optimized by customizing the type of treatment based on the types of cell surface antigens present. We provide comprehensive cytochemical and immunophenotypic characterization of leukemias and lymphomas; B and T cell subset analysis for immunodeficiencies; stem cell counts; solid tumor DNA content and cell cycle analysis.
 
Cytogenetics
Every year, the cytogenetics laboratory processes a heavy volume of neoplastic specimens, utilizing both conventional cytogenetics as well as fluorescence in situ hybridization (FISH). FISH has become the recognized gold standard in determining the likely course of disease and appropriate treatment in leukemias such as chronic lymphocytic.
 
Molecular Biology
Our molecular biology laboratory performs Southern blot hybridization, polymerase chain reaction, and in situ hybridization studies for B and T cell gene rearrangements, many different chromosomal translocations for lymphoma and leukemia, including t(14;18), t(9;22), t(15;17), and Epstein-Barr virus. Many of the molecular tests can be performed on paraffin-embedded tissue.
 
Laboratory accreditation, certification and licensing
The Consultation Services Laboratories at City of Hope are fully accredited by the College of American Pathologists (CAP #22900-01), certified under the Clinical Laboratory Improvements Amendments of 1988 (CLIA '88, ID No. 05D0665695) and licensed by the state of California (CLF 268).

The laboratories participate in the CAP's external proficiency survey program for flow cytometry, immunophenotyping, immunohistochemistry, cytogenetics and molecular oncology. Specific details on our survey performance are available upon request.
 

Antibody Library

Actin
AFP (alpha-fetprotein)
ALK protein
bcl-1
bcl-2
bcl-6
BerEp4
BG8
Calcitonin
Calretinin
CD1
CD2
CD3
CD4
CD5
CD7
CD8
CD10
CD15
CD16
CD20
CD21
CD23
CD30
CD31
CD34
CD35
CD343
CD45RA
CD45/45RB
CD45RO
CD56
CD57
CD68
CD79A
CD99
CD117 (c-kit)
CD138
CEA-Gold1
CEA-Gold5
c-erb-2
Chromogranin
Collagen-IV
DBA.44
Desmin
EBV-LMP
EMA (epithelial membrane  antigen)
ER (estrogen receptor)
Factor XIII (vWF)
FASCIN
GCDFP-15
GFAP
Granzyme B
HBME-1
HCG
Helico
Hemoglobin A
HLA-DR
HMB45
Inhibin
Kappa
Keratin 5/6
Keratin 8/18
Keratin 7
Keratin 8
Keratin 14
Keratin 19 (AE-1)
Keratin 20
Keratin cocktail (pan keratin)
34 βE 12 (HMW Keratin)
Ki-67
Lambda
Lysozyme
Melan-A
mes-13
MOC
MPX (myeloperoxidase)
NF (neurofilament)
p53
PacP (prostate acid phosphatase)
Perforin
PLAP (placential alkaline phosphatase)
PR (progesterone receptor)
Prolactin
PSA (prostate specifice antigen)
S100
SMA (smooth muscle actin)
Surfactant
Synaptophysin
Tdt
TGB (thyroglobin)
Thrombomodulin
TIA-1
TRAP
Tryptase
TTF-1
Villin
Vimentin
vWF (factor VIII)

Specimen Draw and Shipping Requirements

Cytogenetics
Results for STATs, pre-BMT's and newly diagnosed leukemias are available within seven days. All others are available in seven to 10 days but our goal is to report results as soon as they are available. Complex studies may require additional days. Specimens are accepted Monday through Friday, 8 a.m. to 5:30 p.m.
 
Drawing specifications
  • Leukemia and myelodysplastic syndromes: A bone marrow aspirate is the specimen of choice in most cases of leukemia, myelodysplastic and myeloproliferative disorders.
  • Leukemia cytogenetics: Obtain 1 to 2 ml of bone marrow (minimum 0.5 ml) in a heparinized syringe (approximately 500 USP sodium heparin per ml of specimen) and transfer the specimen into a green top sodium heparin tube. Keep at room temperature and ship as soon as possible.
  • Leukemic peripheral blood: Draw 5 to 20 ml of peripheral blood into a green top sodium heparin tube. Mix well by inverting. Keep at room temperature and ship as soon as possible.
  • Lymphoma and solid tumor cytogenetics: Bone marrow core biopsies should be placed in HBSS or RPMI medium.
 
Flow cytometry / molecular diagnostics
The laboratory is open Monday through Friday, 8 a.m. to 5 p.m.
 
Drawing specifications
  • Peripheral blood: For leukemia and lymphoma panels, draw six green top tubes (sodium herapin) and one purple top tube. Maintain at room temperature and ship as soon as possible. ACD tubes are acceptable. Sodium lithium should not be used.
  • Bone marrow: Draw at least one purple top tube, maintain at room temperature and ship as soon as possible. ACD and sodium heparin tubes are acceptable.
 
 

Pathology Team

Pathology Team

Support This Program

It takes the help of a lot of caring people to make hope a reality for our patients.  City of Hope was founded by individuals’ philanthropic efforts 100 years ago. Their efforts — and those of our supporters today — have built the foundation for the care we provide and the research we conduct. It enables City of Hope to strive for new breakthroughs and better therapies, ultimately helping more people enjoy longer, better lives.

For more information on supporting this specific program, please contact our Donor Relations Department at 800-667-5310 or developmentrelations@coh.org. Or, to make a gift that supports all the research at City of Hope, donate online now.
 
We thank you for your support.
 
 
 
Our Programs and Treatments

City of Hope is a national leader in cancer treatment and prevention. Read more...

Our treatment facilities are located throughout our 100+ acre grounds in Duarte, California as well as in  Antelope Valley, South Pasadena, Santa Clarita and Palm Springs.
City of Hope combines compassionate care with the best and most innovative science. Our 100+ acre campus is designed to meet the full range of needs of our patients and families. This guide is designed to help you take advantage of all that is offered at City of Hope - Duarte.
Clinical Trials
Our aggressive pursuit to discover better ways to help patients now – not years from now – places us among the leaders worldwide in the administration of clinical trials.
 
NEWS & UPDATES
  • Brain tumor removal would seem to be the obvious course of action in the wake of a brain tumor diagnosis, but that’s not always the case. Some tumors are too difficult for many surgeons to reach or too close to areas that control vital functions. Removing them just proves too risky. A new device at City [...
  • Hijacking the same sorts of viruses that cause HIV and using them to reprogram immune cells to fight cancer sounds like stuff of the future. Some scientists believe that the future is closer than we think – and are now studying the approach in clinical trials at City of Hope. Immunotherapy is a promising approa...
  • Nausea is the one of the most well-known, and dreaded, side effects of cancer treatment — and with good reason. Beyond the quality-of-life issues that it causes, severe nausea can prevent patients from receiving enough nutrients and calories at a time when they need every edge they can get. A few simple actions...
  • With Labor Day just around the corner, summer is on its way out. But just because summertime is ending doesn’t mean we can skip sunscreen. Protection from ultraviolet (UV) radiation is needed all year round. Exposure to UV radiation — whether from the sun or from artificial sources such as sunlamps used i...
  • Undergoing reconstructive surgery may seem like a forgone conclusion for survivors of breast cancer, but that doesn’t appear to be the case. A new study has found that most breast cancer survivors who undergo a mastectomy decide against surgical reconstruction of their breasts. The reasons for such a deci...
  • Nearly four decades ago, City of Hope began its bone marrow transplant program. Its first transplant reunion celebration was a single patient and his donor, also his brother. This year, City of Hope welcomed hundreds of hematopoietic cell transplant (HCT) recipients to the annual bone marrow transplant/HCT reun...
  • The burgeoning type 2 diabetes epidemic casts a pall over the health of America’s public. New research now shows the looming threat is getting worse. Much worse. A diabetes trends study published earlier this month in the Lancet Diabetes and Endocrinology by researchers at the federal Centers for Disease Contro...
  • An aspirin a day might help keep breast cancer away for some breast cancer survivors, a new study suggests. Obese women who have had breast cancer could cut their risk of a recurrence in half if they regularly take aspirin or other nonsteroidal anti-inflammatory drugs, called NSAIDs, report researchers from the...
  • Christine Crews isn’t only a fitness enthusiast, she’s also a personal trainer and fitness instructor. Being active defines her life. So when she was diagnosed with bladder cancer at age 30, she decided she absolutely couldn’t let the disease interfere with that lifestyle. And it didn’t. For t...
  • Cancer treatment and the cancer itself can cause changes in your sense of taste or smell. These side effects typically subside after treatment ends, but there are ways to help alleviate those bitter and metallic tastes in your mouth. Here are tips from the National Cancer Institute to help keeps tastes and food...
  • Immunotherapy — using one’s immune system to treat a disease — has been long lauded as the “magic bullet” of cancer treatments, one that can be more effective than the conventional therapies of surgery, radiation or chemotherapy. One specific type of immunotherapy, called adoptive T cell thera...
  • Today, when cancer spreads from its original site to other parts of the body, a process known as metastasis, patients face an uphill battle. Treatments are poorly effective, and cures are nearly impossible. Further, incidence rates for these types of cancers are increasing – particularly for cancers that have s...
  • Thanks to the California Institute for Regenerative Medicine (CIRM), high school students across the state gained valuable hands-on experience with stem cell research this summer. City of Hope hosted eight of those students. As part of the CIRM Creativity Awards program, the young scholars worked full time as m...
  • Radiation therapy can help cure many children facing Hodgkin lymphoma and other cancers. When the radiation is delivered to a girl’s chest, however, it can lead to a marked increase in breast cancer risk later in life. A recent multi-institutional study that included City of Hope’s Smita Bhatia, M.D., M.P.H., t...
  • A patient diagnosed with cancer – especially a rare, advanced or hard-to-treat cancer – needs specialized care from exceptionally skilled and highly trained experts. That kind of care saves lives, improves quality of life and keeps families whole. That kind of care is best found at comprehensive cancer centers ...