Contact Information
John C. Williams, Ph.D.
  • Associate Professor, Molecular Medicine
  • Director, X-ray Crystallography Core Facility
  • Co-director, Drug Discovery and Structural Biology (DDSB) Core

Departments/Division/Program

Professional Experience


Professional Experience

  • 2008 - present, Assistant Professor, Department of Molecular Medicine, Beckman Research Institute of City of Hope, Duarte, CA
  • 2008 - present, Director, X-ray Crystallography Core Facility, Beckman Research Institute of City of Hope, Duarte, CA
  • 2005 - 2008, Co-director, Small Molecule Screen Core, Kimmel Cancer Center, Philadelphia, PA
  • 2005 - 2008, Director, X-ray Core Facility, Kimmel Cancer Center, Philadelphia, PA
  • 2004 - 2008, Assistant Professor, Thomas Jefferson University, New York, NY
  • 2002 - 2004, Associate Research Scientist, Columbia University, New York, NY
  • 2001 - 2004, Associate Research Scientist, Osborne Marine Sciences Lab, New York Aquarium, Brooklyn, NY
 

Education

 
Education/Training
  • 1999 - 2002, Postdoctoral Fellow, Department of Biochemistry and Molecular Biophysics, Columbia University
  • 1999 - 2002, Special Fellow, Leukemia Society of America
  • 1997 - 1998, Howard Hughes Medical Institute Research Associate
  • 1995 - 1997, Alexander von Humboldt Fellow, European Molecular Biology Laboratory, Heidelberg, Germany
  • 1995, Columbia University, M.S., Ph.D., Chemistry
  • 1993, Columbia University, M.Phil., Chemistry
  • 1990, University of Californa, Santa Cruz, B.S., Chemistry
 

Honors/Awards

 
Awards
  • 1989, Elizabeth Renald Memorial Scholarship

Honors
  • 1995 - 1997, Alexander Von Humboldt Fellow
  • 1997 - 1998, Howard Hughes Medical Institute Fellow
  • 1990, Departmental Honors, UC Santa Cruz
 

Memberships


  • 2001, American Society of Cell Biology

Publications


Selected Publications
 
Yan S, Hou G, Schwieters CD, Ahmed S, Williams JC, Polenova T. Three-dimensional structure of CAP-gly domain of mammalian dynactin determined by magic angle spinning NMR spectroscopy: conformational plasticity and interactions with end-binding protein EB1. J Mol Biol. (2013). 425(22):4249-66
 
Donaldson JM, Zer C, Avery KN, Bzymek KP, Horne DA, Williams JC. Identification and grafting of a unique peptide-binding site in the Fab framework of monoclonal antibodies. Proc Natl Acad Sci U.S.A. (2013). 110(43):17456-61
 
Siglin AE, Sun S, Moore JK, Tan S, Poenie M, Lear JD, Polenova T, Cooper JA, Williams JC. Dynein and dynactin leverage their bivalent character to form a high-affinity interaction. PLOS One (2013). 8(4):e59453
 
Sun S, Yan S, Guo C, Li M, Hoch JC, Williams JC, Polenova T. A time-saving strategy for MAS NMR spectroscopy by combining nonuniform samling and paramagnetic relaxation assisted condensed data collection. J Phys Chem B. (2012). 116(46)L13585-96
 
Sun S, Han Y, Paramasivam S, Yan S, Siglin AE, Williams JC, Byeon IJ, Ahn J, Gronenborn AM, Polenova T. Solid-state NMR spectroscopy of protein complexes. Methods Mol Biol. (2012). 831:303-31
 
Stuchell-Brereton MD, Siglin A, Li J, Moore JK, Ahmed S, Williams JC, Cooper JA. Functional interaction between dynein light chain and intermediate chain is required for mitotic spindle positioning. Mol Biol Cell. (2011). 22(15):2690-701
 
Sakurai N, Maeda M, Lee SU, Ishikawa Y, Li M, Williams JC, Wang L, Su L, Suzuki M, Saito TI, Chiba S, Casola S, Yagita H, Teruya-Feldstein J, Tsuzuki S, Bhatia R, Maeda T. The LRF transcription factor regulates mature B cell development and the germinal center response in mice. J Clin Invest. (2011). 121(7):2583-98
 
Buettner R, Corzano R, Rashid R, Lin J, Senthil M, Hedvat M, Schroeder A, Mao A, Herrmann A, Yim J, Li H, Yuan YC, Yakushijin K, Yakushijin F, Vaidehi N, Moore R, Gugiu G, Lee TD, Yip R, Chen Y, Jove R, Horne D, Williams JC. Alkylation of cysteine 468 in Stat3 defines a novel site for therapeutic development. ACS Chem Biol. (2011). 6(5):432-43
 
Sun S, Butterworth AH, Paramasivam S, Yan S, Lightcap CM, Williams JC, Polenova T. Resonance Assignments and Secondary Structure Analysis of Dynein Light Chain 8 by Magic Angle Spinning NMR Spectroscopy. Can J Chem. (2011). 89(7):909-918
 
Ahmed S, Sun S, Siglin AE, Polenova T, Williams JC. Disease-associated mutations in the p150(Glued) subunit destabilize the CAP-gly domain. Biochemistry. (2010). 49(25):5083-5
 
Varma D, Dawn A, Ghosh-Roy A, Weil SJ, Ori-McKenney KM, Zhao Y, Keen J, Vallee RB, Williams JC. Development and pplication of in vivo molecular traps reveals that dynein light chain occupancy differentially affects dynein-mediated processes. Proc Natl Acad Sci U.S.A. (2010). 107(8):3493-8
 
Donaldson JM, Kari C, Fragoso RC, Rodeck U, Williams JC. Design and development of masked therapeutic antibodies to limit off-target effects: application to anti-EGFR antibodies. Cancer Biol Ther. (2009). 8(22):2147-52
 
Kang DS, Kern RC, Puthenveedu MA, von Zastrow M, Williams JC, Benovic JL. Structure of an arrestin2-clathrin complex reveals a novel clathrin binding domain that modulates receptor trafficking. J Biol Chem. (2009). 284(43):29860-72
 
Sun S, Siglin A, Williams JC, Polenova T. Solid-state and solution NMR studies of the CAP-Gly domain of mammalian dynactin and its interaction with microtubules. J Am Chem Soc. (2009) 131(29):10113-26
 
Lightcap CM, Kari G, Arias-Romero LE, Chernoff J, Rodeck U, Williams JC. interaction with LC8 is required for Pak1 nuclear import and is indispensable for zebrafish development. PLOS One. (2009) 4(6):e6025
 
Höök P, Yagi T, Ghos-Roy A, Williams JC, Vallee RB. The dynein stalk contains an antiparallel coiled coil with region-specific stability. Biochemistry. (2009) 48(12):2710-3
 
Rodeck U, Williams JC. Nature's design  a blueprint for engineered therapeutic antibodies, Cancer Biol Ther. (2008) 7(11): 1746-1747

Lightcap CM, Sun S, Lear JD, Rodeck U, Polenova T, Williams JC. Biochemical and Structural Characterization of the Pak1-LC8 Interaction. J Biol Chem. (2008) 283(40): 27314-24

Kamat V, Donaldson JM, Kari C, Quadros MR, Lelkes PI, Chaiken I, Cocklin S, Williams JC, Papazoglou E, Rodeck U. Enhanced EGFR inhibition and distinct epitope recognition by EGFR antagonistic mAbs C225 and 425. Cancer Biol Ther. (2008) 7(5):726-33

Williams, JC, Roulhak, P, Roy AG, Vallee RB, Fitzgerald, MC & Hendrickson WA Complex of LC8 and TcTex1 to dynein intermediate chain suggests regulatory role for dynein motor function, Proc Natl Acad Sci U S A. (2007) 104(24):10028-33

Tan, GS, Pruess, MAR, Williams, JC, Schnell, MJ: The Dynein Light Chain 8 Binding motif of Rabsi Virus Phosphoprotein promotes Efficient Viral Transcription, Proc Natl Acad Sci U S A. (2007). 104(17):7229-34

Williams, JC, Xie, H & Hendrickson, WA, Crystal structure of dynein light chain TcTex-1, J Biol Chem. (2005) 280(23):21981-6.

Williams JC, Sue C, Banting GS, Yang H, Glerum DM, Hendrickson WA, Schon EA. Crystal structure of human SCO1: Implications for redox signalingby a mitochondrial cytochrome c oxidase  "assembly" protein. J Biol Chem. (2005) 280(15):15202-11
 
Vallee, RB, Williams, JC, Varma D, & Barnhart LE: Dynein, an Ancient Motor Protein Involved in Multiple Modes of Transport. J. Neurobiol. (2004) 8(2): 189-200

Willams JC, Zeelen JP, Neubauer G, Vriend G, Backmann J, Michels PAM, Lambeir AM, Wierenga RK: Structural and mutagenesis studies of leishmania triosephosphate isomerase: a point mutation can convert a mesophilic enzyme into a superstable enzyme without losing catalytic power. Protein Engineering. (1999) 12: 243-250

Williams JC, Wierenga RK, Saraste M: Insights into Src Kinase functions: Structural comparisons. Trends In Biochemical Science (1998) 23: 179-184

Williams JC, McDermott AE: Variable NMR Spin-Lattice Relaxation Times in Secondary Amides: Effect of Ramachandran Angles on the Librational Dynamics. J. Phys. Chem. (1998) 102: 6248-6259

Liu K, Williams JC, Goodin DB, McDermott AE: Solid State Deuterium NMR of  Imidazole Ligands in Cytochrome c Peroxidase. J. Am. Chem. Soc. (1998) 120: 10199- 10202

Williams JC, Weijland A, Gonfloni S, Thompson A, Courtneidge SA, Superti-Furga G,Wierenga, RK: The 2.35Å Crystal Structure of chicken Src, A dynamic molecule regulated by multipoint interaction. J. Mol. Biol.  (1997) 274: 757-775

Gonfloni S, Williams JC, Hattula K, Wiejland A, Wierenga RK, Superti-Furga G: The role of the linker between the SH2 domain and the catalytic domain in the regulation and function of Src. EMBO J. (1997) 16: 7261-7271
  
Weijland A, Williams JC, Neubauer G, Courtneidge SA, Wierenga RK, Superti-Furga G: Src regulated by C-terminal phosphorylation is monomeric. PNAS (1997) 94:3590-3595

Palmer AG, Williams JC, McDermott AE: Nuclear Magnetic Resonance Studies of Biopolymer Dynamics. J. Phys. Chem. (1996) 100: 13293-13310

Williams JC, McDermott, AE: Dynamics of the Flexible Loop in  Isomerase: Loop Motion is not Ligand Gated. Biochemistry (1995) 34: 8309-8319

Copié V, McDermott AE, Beshah K, Williams JC, Spijker-Assink M, Gebhard R, Lugtenberg J, Herzfeld J, Griffin RG: Deuterium Solid-State NMR Studies of  Methyl Group Dynamics in Bacteriorhodopsin and Retinal Model Compounds:  Evidence of a 6-s-Trans Chromophore in the Protein. Biochemistry (1994) 33:3280- 3286

Williams JC, McDermott AE: Cis-Trans Energetics in Urea and Acetamide Studied by Deuterium NMR. J. Phys. Chem. (1993) 97:12393-12398

Locations


  • City of Hope - Main Campus (Duarte)
    1500 East Duarte Road
    Duarte, CA 91010