A National Cancer Institute-designated Comprehensive Cancer Center

Make an appointment: 800-826-HOPE
Light Microscopy Digital Imaging Bookmark and Share

Light Microscopy Digital Imaging

The objective of the Light Microscopy Digital Imaging Core is to provide services, facilities and expertise to City of Hope investigators needing high quality light microscope images.

Microscopy and imaging technology are rapidly evolving fields that depend increasingly on expensive equipment and skills beyond the means of most individual laboratories. We seek to provide a range of research-grade light and confocal microscope platforms for simple observations and documentation suitable for publication, education or grant proposals.

The Light Microscopy Digital Imaging Core also makes available the latest research platforms for automated microscopy experiments, advanced observation techniques and sophisticated digital image processing and analysis.

Note: This core is not supported by the National Cancer Institute-funded Cancer Center Support Grant.


Services
  • Instruction in general aspects of light and confocal microscopy
  • Instruction in general aspects of digital imaging and image analysis
  • Instruction in operation of Light Microscopy Digital Imaging Core equipment
  • Consultation on microscopy and imaging projects
 
Microscopes
 

Last updated: 2013
 

Abstract for Grants

The objective of the City of Hope Light Microscopy Digital Imaging Core is to provide service, facilities and expertise to all City of Hope and Beckman Research Institute investigators who need high quality light microscopy images. The facility also seeks to provide state-of-the-art research platforms for automated microscopy experiments, advanced observation techniques and advanced digital image processing and analysis.
 
The Light Microscopy Digital Imaging Core has the following widefield light microscopes: Zeiss Observer Z1 for live-cell automated imaging in fluorescent or color imaging modes, Zeiss AxioVert 200 for fluorescent applications, Olympus AX70 upright for slide mounted specimens, Olympus IX81inverted for culture dish specimens, and an Olympus BX50 upright for fluorescent imaging. The facility also has confocal microscopes that include: Zeiss LSM510 2-Photon upright microscope, LSM510 META 2-Photon inverted microscope, LSM700 inverted microscope, and a Prairie Technologies Ultima 2-Photon microscope using a Coherent Chameleon Ultra II laser. The Core also has a Leica LMD7000 microscope system used for LASER MicroDissection and microirradiation experiments.
 
 
The Light Microscopy Digital Imaging Core is located in the Furth Building, Rooms F1113, F1114, and F1101.
 

Imaging Software

  • AutoQuant, deconvolution software
  • Media Cybernetics, Image Pro Premier high-end image analysis software
  • Adobe Photoshop
  • Amira 3D Projection and Analysis software
  • ZEN 2012
  • Image J / FIJI
 

Imaging Station

For demanding imaging applications such as 3D rendering, quantification, and analysis we provide a high-end imaging workstation.

Dell Precision T3500, Intel Xeon CPU, Windows 7 64-bit OS, 15GB RAM, and NVIDIA Quadro FX 5800 Video Card.

Programs include: Amira, Image Pro Premier, Zen 2012, LAS AF lite, MIPAV, Graph Pad, Photoshop CS5, Image J, FIJI, Reconstruct, Cell Profiler, Image Scope, and Blender.

 

Teaching Station

This station has a wheeled cart with wireless keyboard and mouse that can operate the computer from anywhere in the room. Computer is Dell Optiplex 7010 and wall mounted monitor is a 50" Sharp Plasma. This computer is networked and has Image Pro Premier as well as other imaging programs installed.

LabWare File Management System

How to download Zen Lite 2012 Image Browser to view your images

  • Click on the link provided below.
  • http://microscopy.zeiss.com/microscopy/en_de/products/microscope-software/zen-lite.html#inpagetabs-0
  • Once you click on the link, you will be directed to the Zeiss Webpage.
  • Select the tab “Downloads”
  • Once in the “downloads” tab, locate and select “Register to download your copy of Zen lite free of charge” or you can click on the link provided to take you straight to the webpage. Register to download your copy of ZEN lite free of charge.
  • It will take you to a screen like the one below. Enter your information in the “Required Data” area.        
     
  • Select the “submit” button at the bottom of your screen.
  • An email will be sent with a download link to the email you provided.
  • Once you select the download link, you will be taken to the webpage (See below)
  • Choose the appropriate version (32-bit or 64-bit) for your computer by selecting the “Download via HTTP-protocol”             
  • Once you select the appropriate version, a window will appear prompting you to select “run” or “save.” Select “Save”
                           
  • Name and Save the file in an appropriate folder.
  • A window will appear. Once it says “download complete,” select the “run” button.
  • You will be presented with a download wizard to install the program.
  • Follow the step by step instructions until the download is complete.
  • Zen Lite 2012 will now be available on your computer or desktop.
 

Scheduling Equipment

In order to use the Light Microscopy Digital Imaging Core, you must receive training from the staff on the system you would like to use. Please contact us via email for training. All City of Hope users can reserve microscope times online through the iLab Solutions website (coh.ilabsolutions.com). Non-City of Hope users should contact us to reserve microscope time. Please contact us if you have any questions.
 
  • LSM510 Upright
  • LSM510 Inverted
  • LSM700 Inverted
  • Prairie Ultima 2-Photon
 
LASER MicroDissection
  • Leica LMD7000
 
  • Zeiss Observer Z1
  • Zeiss AxioVert 200
  • 1X81 Inverted
  • AX70 Upright
  • BX50 Upright
 

Pricing

Current service offering and pricing can be found on our iLab site. Please contact us for further questions.

Contact Us

Location:
Light Microscopy Digital Imaging Core   
City of Hope
1500 East Duarte Road
Furth Building
Room F1113
Duarte, CA 91010-3000

626-301-8870 phone
626-301-8908 fax

Light Microscopy Digital Imaging

Light Microscopy Digital Imaging

The objective of the Light Microscopy Digital Imaging Core is to provide services, facilities and expertise to City of Hope investigators needing high quality light microscope images.

Microscopy and imaging technology are rapidly evolving fields that depend increasingly on expensive equipment and skills beyond the means of most individual laboratories. We seek to provide a range of research-grade light and confocal microscope platforms for simple observations and documentation suitable for publication, education or grant proposals.

The Light Microscopy Digital Imaging Core also makes available the latest research platforms for automated microscopy experiments, advanced observation techniques and sophisticated digital image processing and analysis.

Note: This core is not supported by the National Cancer Institute-funded Cancer Center Support Grant.


Services
  • Instruction in general aspects of light and confocal microscopy
  • Instruction in general aspects of digital imaging and image analysis
  • Instruction in operation of Light Microscopy Digital Imaging Core equipment
  • Consultation on microscopy and imaging projects
 
Microscopes
 

Last updated: 2013
 

Abstract for Grants

Abstract for Grants

The objective of the City of Hope Light Microscopy Digital Imaging Core is to provide service, facilities and expertise to all City of Hope and Beckman Research Institute investigators who need high quality light microscopy images. The facility also seeks to provide state-of-the-art research platforms for automated microscopy experiments, advanced observation techniques and advanced digital image processing and analysis.
 
The Light Microscopy Digital Imaging Core has the following widefield light microscopes: Zeiss Observer Z1 for live-cell automated imaging in fluorescent or color imaging modes, Zeiss AxioVert 200 for fluorescent applications, Olympus AX70 upright for slide mounted specimens, Olympus IX81inverted for culture dish specimens, and an Olympus BX50 upright for fluorescent imaging. The facility also has confocal microscopes that include: Zeiss LSM510 2-Photon upright microscope, LSM510 META 2-Photon inverted microscope, LSM700 inverted microscope, and a Prairie Technologies Ultima 2-Photon microscope using a Coherent Chameleon Ultra II laser. The Core also has a Leica LMD7000 microscope system used for LASER MicroDissection and microirradiation experiments.
 
 
The Light Microscopy Digital Imaging Core is located in the Furth Building, Rooms F1113, F1114, and F1101.
 

Imaging Software

Imaging Software

  • AutoQuant, deconvolution software
  • Media Cybernetics, Image Pro Premier high-end image analysis software
  • Adobe Photoshop
  • Amira 3D Projection and Analysis software
  • ZEN 2012
  • Image J / FIJI
 

Imaging Station

For demanding imaging applications such as 3D rendering, quantification, and analysis we provide a high-end imaging workstation.

Dell Precision T3500, Intel Xeon CPU, Windows 7 64-bit OS, 15GB RAM, and NVIDIA Quadro FX 5800 Video Card.

Programs include: Amira, Image Pro Premier, Zen 2012, LAS AF lite, MIPAV, Graph Pad, Photoshop CS5, Image J, FIJI, Reconstruct, Cell Profiler, Image Scope, and Blender.

 

Teaching Station

This station has a wheeled cart with wireless keyboard and mouse that can operate the computer from anywhere in the room. Computer is Dell Optiplex 7010 and wall mounted monitor is a 50" Sharp Plasma. This computer is networked and has Image Pro Premier as well as other imaging programs installed.

LabWare File Management System

How to download Zen Lite 2012 Image Browser to view your images

  • Click on the link provided below.
  • http://microscopy.zeiss.com/microscopy/en_de/products/microscope-software/zen-lite.html#inpagetabs-0
  • Once you click on the link, you will be directed to the Zeiss Webpage.
  • Select the tab “Downloads”
  • Once in the “downloads” tab, locate and select “Register to download your copy of Zen lite free of charge” or you can click on the link provided to take you straight to the webpage. Register to download your copy of ZEN lite free of charge.
  • It will take you to a screen like the one below. Enter your information in the “Required Data” area.        
     
  • Select the “submit” button at the bottom of your screen.
  • An email will be sent with a download link to the email you provided.
  • Once you select the download link, you will be taken to the webpage (See below)
  • Choose the appropriate version (32-bit or 64-bit) for your computer by selecting the “Download via HTTP-protocol”             
  • Once you select the appropriate version, a window will appear prompting you to select “run” or “save.” Select “Save”
                           
  • Name and Save the file in an appropriate folder.
  • A window will appear. Once it says “download complete,” select the “run” button.
  • You will be presented with a download wizard to install the program.
  • Follow the step by step instructions until the download is complete.
  • Zen Lite 2012 will now be available on your computer or desktop.
 

Scheduling Equipment

Scheduling Equipment

In order to use the Light Microscopy Digital Imaging Core, you must receive training from the staff on the system you would like to use. Please contact us via email for training. All City of Hope users can reserve microscope times online through the iLab Solutions website (coh.ilabsolutions.com). Non-City of Hope users should contact us to reserve microscope time. Please contact us if you have any questions.
 
  • LSM510 Upright
  • LSM510 Inverted
  • LSM700 Inverted
  • Prairie Ultima 2-Photon
 
LASER MicroDissection
  • Leica LMD7000
 
  • Zeiss Observer Z1
  • Zeiss AxioVert 200
  • 1X81 Inverted
  • AX70 Upright
  • BX50 Upright
 

Pricing

Pricing

Current service offering and pricing can be found on our iLab site. Please contact us for further questions.

Contact Us

Contact Us

Location:
Light Microscopy Digital Imaging Core   
City of Hope
1500 East Duarte Road
Furth Building
Room F1113
Duarte, CA 91010-3000

626-301-8870 phone
626-301-8908 fax
City of Hope
Light Microscopy Digital Imaging Core
1500 East Duarte Road
Furth Building
Room F1113
Duarte, CA 91010
626-301-8870 phone
626-301-8908 fax
DIC Striated Muscle
Click here for more information on our Widefield Light Microscopes.
Developing fruit fly nervous system
Click here for more information on our Confocal Microscopes.
Research Shared Services

City of Hope embodies the spirit of scientific collaboration by sharing services and core facilities with colleagues here and around the world.
 

Beckman Research Institute of City of Hope is internationally  recognized for its innovative biomedical research.
City of Hope is one of only 41 Comprehensive Cancer Centers in the country, the highest designation awarded by the National Cancer Institute to institutions that lead the way in cancer research, treatment, prevention and professional education.


NEWS & UPDATES
  • The physical side effects of cancer can damage anyone’s self-confidence, but especially that of women who, rightly or wrongly, are more likely to find their appearance (or their own perception of their appearance) directly connected to their ability to face the world with something resembling ap...
  • The promise of stem cell therapy has long been studied in laboratories. Now, as medicine enters an era in which this therapy will be increasingly available to patients, the nurses who help deliver it will be in the spotlight. City of Hope, which has launched its Alpha Clinic for Cell Therapy and Innovation (ACT...
  • Just because you can treat a condition, such as high cholesterol, at the end of life — well, that doesn’t mean you should. That’s the basic lesson of a study to be published March 30 in JAMA Internal Medicine. The ramifications go far beyond that. The research, in which City of Hope’s Betty Fe...
  • The understanding of the relationship between genetics and cancer risk continues to grow, with more genetic testing than ever before available to patients. However, the adage that a little knowledge is a dangerous thing is applicable: Without context for what a test result means, and without meaningful guidance...
  • Standard prostate biopsies haven’t changed significantly in the past 30 years – nor have the problems inherent with them. Regular biopsies have an expected error rate: Tumors may potentially be undersampled and, 30 percent of the time, men who undergo a radical prostatectomy are found to have more aggress...
  • In the field of cancer, patients have had surgery, chemotherapy and radiation therapy as options. Now, as City of Hope officially opens the Alpha Clinic for Cellular Therapy and Innovation, patients battling cancer and other life-threatening diseases have another option: stem-cell-based therapy. The Alpha Clini...
  • How does the environment affect our health? Specifically, how does it affect our risk of cancer? City of Hope physicians and researchers recently answered those questions in an Ask the Experts event in Corona, California, explaining the underlying facts about how the environment can affect our health. Moderator...
  • Nurses and other medical professionals have come to understand that it’s not enough just to fight disease. They also must provide pain relief, symptom control, and an unrelenting commitment to improve patients’ quality of life — especially at the end of life. Not too long ago, this was a relatively ...
  • “Tonight, I’m launching a new precision medicine initiative to bring us closer to curing diseases like cancer.” These were the words of President Barack Obama on Jan. 20, 2015, during his State of the Union address. So what is precision medicine, and how close are we to making it a reality for...
  • March is Colon Cancer Awareness Month. How sad, yet how serendipitous, that the co-creator of “The Simpsons” Sam Simon passed away in March after a four-year battle against colon cancer. What message can we all learn from his illness that can help us prevent and overcome colon cancer in our own lives? Colon can...
  • Misagh Karimi, M.D., assistant clinical professor, is a medical oncologist at one of City of Hope’s newest community practice locations, located in Corona in Riverside County. A recent community health report from Corona’s public health department stated that obesity rates for teens and adults in Riverside Coun...
  • In 1975, the median survival for patients with ovarian cancer was about 12 months. Today, the median survival is more than 5 years. Although researchers and clinicians are far from satisfied, the progress in ovarian cancer treatment is encouraging, said Robert Morgan, M.D., F.A.C.P., professor of medical oncolo...
  • Colorectal cancer may be one of the most common cancers in both men and women, but it’s also one of the most curable cancers. Today, because of effective screening tests and more advanced treatment options, there are more than 1 million survivors of colorectal cancer in the United States. Here, colorectal...
  • Breast cancer treatment can damage a woman’s ability to become pregnant, making the impact on fertility one of the key factors that many consider when choosing a therapy regimen. Now a study has found that breast cancer patients treated with a hormone-blocking drug in addition to chemotherapy were less li...
  • My colleagues in the clinic know I’ve got a soft spot. Last week, a patient of mine offered me a fantastic compliment. “You’re looking younger these days, Dr. Pal!” she said, offering me a big hug as she proceeded out of the clinic room. Lovely, I thought. The early morning workouts are paying off. She continue...