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Light Microscopy Digital Imaging

Light Microscopy Digital Imaging
The objective of the Light Microscopy Digital Imaging Core is to provide services, facilities and expertise to City of Hope investigators needing high quality light microscope images.

Microscopy and imaging technology are rapidly evolving fields that depend increasingly on expensive equipment and skills beyond the means of most individual laboratories. We seek to provide a range of research-grade light and confocal microscope platforms for simple observations and documentation suitable for publication, education or grant proposals.

The Light Microscopy Digital Imaging Core also makes available the latest research platforms for automated microscopy experiments, advanced observation techniques and sophisticated digital image processing and analysis.

Note: This core is not supported by the National Cancer Institute-funded Cancer Center Support Grant.


Services
  • Instruction in general aspects of light and confocal microscopy
  • Instruction in general aspects of digital imaging and image analysis
  • Instruction in operation of Light Microscopy Digital Imaging Core equipment
  • Consultation on microscopy and imaging projects
 
Microscopes
 

Last updated: 2013
 

Abstract for Grants

The objective of the City of Hope Light Microscopy Digital Imaging Core is to provide service, facilities and expertise to all City of Hope and Beckman Research Institute investigators who need high quality light microscopy images. The facility also seeks to provide state-of-the-art research platforms for automated microscopy experiments, advanced observation techniques and advanced digital image processing and analysis.
 
The Light Microscopy Digital Imaging Core has the following widefield light microscopes: Zeiss Observer Z1 for live-cell automated imaging in fluorescent or color imaging modes, Zeiss AxioVert 200 for fluorescent applications, Olympus AX70 upright for slide mounted specimens, Olympus IX81inverted for culture dish specimens, and an Olympus BX50 upright for fluorescent imaging. The facility also has confocal microscopes that include: LSM510 2-Photon upright microscope, LSM510 META 2-Photon inverted microscope, LSM700 inverted microscope, and a Prairie Technologies Ultima 2-Photon microscope using a Coherent Chameleon Ultra II laser.
 
 
The Light Microscopy Digital Imaging Core is located in the Furth Building, Rooms F1113, F1114, and F1101, and the Prairie Microscope is located in Familian F1101A.
 

Imaging Software

  • AutoQuant, deconvolution software
  • Media Cybernetics, Image Pro Premier high-end image analysis software
  • Adobe Photoshop
  • Amira 3D Projection and Analysis software
  • AxioVision 4.8.2
  • Image J / Fiji
  • Zen 2012
 

Imaging Station

For demanding imaging applications such as 3D rendering, quantification, and analysis we provide a high-end imaging station.

Dell Precision T3500, Intel Xeon CPU, Windows 7 64-bit OS, 15GB RAM, and NVIDIA Quadro FX 5800 Video Card.

Programs include: Amira, Image Pro Premier, Zen 2012, LAS AF lite, MIPAV, Graph Pad, Photoshop CS5, Image J, FIJI, Reconstruct, Cell Profiler, Image Scope, and Blender.

 

Teaching Station

This station has a wheeled cart with wireless keyboard and mouse that can operate the computer from anywhere in the room. Computer is Dell Optiplex 7010 and wall mounted monitor is a 50" Sharp Plasma. This computer is networked and has Image Pro Premier as well as other imaging programs installed.
 

How to download Zen Lite 2012 Image Browser to view your images

  • Click on the link provided below.
  • http://microscopy.zeiss.com/microscopy/en_de/products/microscope-software/zen-lite.html#inpagetabs-0
  • Once you click on the link, you will be directed to the Zeiss Webpage.
  • Select the tab “Downloads”
  • Once in the “downloads” tab, locate and select “Register to download your copy of Zen lite free of charge” or you can click on the link provided to take you straight to the webpage. Register to download your copy of ZEN lite free of charge.
  • It will take you to a screen like the one below. Enter your information in the “Required Data” area.        
     
  • Select the “submit” button at the bottom of your screen.
  • An email will be sent with a download link to the email you provided.
  • Once you select the download link, you will be taken to the webpage (See below)
  • Choose the appropriate version (32-bit or 64-bit) for your computer by selecting the “Download via HTTP-protocol”             
  • Once you select the appropriate version, a window will appear prompting you to select “run” or “save.” Select “Save”
                           
  • Name and Save the file in an appropriate folder.
  • A window will appear. Once it says “download complete,” select the “run” button.
  • You will be presented with a download wizard to install the program.
  • Follow the step by step instructions until the download is complete.
  • Zen Lite 2012 will now be available on your computer or desktop.
 

Scheduling Equipment

In order to use the Light Microscopy Digital Imaging Core, you must receive training from the staff on the system you would like to use. Please contact us for training. All City of Hope users can reserve microscope times online through the Microsoft Outlook Calendar. Non-City of Hope certified users should contact us to reserve microscope time. Please contact us if you have any questions.
 
  • LSM510 Upright: MS Outlook/All Public Folders/Neurosciences/Confocal/LSM 510 Upright 2 Photon
  • LSM510 Inverted: MS Outlook/All Public Folders/Neurosciences/Confocal/LSM 510 Meta Inverted 2 Photon
  • LSM700 Inverted: MS Outlook/All Public Folders/Neurosciences/Confocal/LSM 700
  • Prairie Ultima 2-Photon: MS Outlook/All Public Folders/Neurosciences/Confocal/Prairie
 
  • Zeiss Observer: MS Outlook/All Public Folders/Neurosciences/Microscopy Core/Observer
  • Zeiss AxioVert 200: MS Outlook/All Public Folders/Neurosciences/Microscopy Core/AxioVert
  • 1X81 Inverted: MS Outlook/All Public Folders/Neurosciences/Microscopy Core/Inverted IX81
  • AX70 Upright: MS Outlook/All Public Folders/Neurosciences/Microscopy Core/AX70
  • BX50 Upright Mono: MS Outlook/All Public Folders/Neurosciences/Microscopy Core/Bx50
 

Pricing

Service                                           User Rates

Confocal microscopy                   $75.00/hr 

Widefield microscopy                  $15.00/hr 

If you are a City of Hope employee, please click here for pricing.

Contact Us

Location:
Light Microscopy Digital Imaging Core   
City of Hope
1500 East Duarte Road
Furth Building
Room F1113
Duarte, CA 91010-3000

626-301-8870 phone
626-301-8908 fax
City of Hope
Light Microscopy Digital Imaging Core
1500 East Duarte Road
Furth Building
Room F1113
Duarte, CA 91010
626-301-8870 phone
626-301-8908 fax
DIC Striated Muscle
Click here for more information on our Widefield Light Microscopes.
Developing fruit fly nervous system
Click here for more information on our Confocal Microscopes.
Research Shared Services

City of Hope embodies the spirit of scientific collaboration by sharing services and core facilities with colleagues here and around the world.
 

Beckman Research Institute of City of Hope is internationally  recognized for its innovative biomedical research.
City of Hope is one of only 41 Comprehensive Cancer Centers in the country, the highest designation awarded by the National Cancer Institute to institutions that lead the way in cancer research, treatment, prevention and professional education.
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